Study Identifies Potential Genes Promoting Prostate Cancer Metastasis

A study identified and validated genes that play a role in the functionality for the growth and metastasis of prostate cancer. The research appeared in Current Cancer Drug Targets.

In this study, researchers developed DU145-KO cell line by transfecting DU145 cells with lentivirus packaged with the genome-wide knockout library. Subsequently DU145-KO cells were transplanted into the armpits of immunocompromise mice. The investigators followed by collating lung samples at week 3 (early lung tissue), or week 7 (late lung tissue with micro-metastasis), as well as from primary tumor site at week 7 (late primary tumor). Moreover, the researchers recorded lung metastasis at various points of gene sequencing.

According to the results, DU145-KO cells promoted the formation of transplanted tumors in mice, as well as the growth and metastasis of primary tumors, compared to the control arm of DU145-NC cells. The study identified fifteen target genes(C1QTNF9B, FAM229A, hsa-mir-3929, KRT23, TARS2, CRADD, GRIK4, PLA2G15, LOXL1, SLITRK6, CDC42EP5, SLC2A4, PTGDS, MYL9 and ACOX2) for validation, which demonstrated that knockout of any of these genes resulted in enhanced potential of metastasis in DU145 cells.

“Genome-wide CRISPR-Cas9 knockout screening technology combined with high-throughput sequencing analysis identified genes that potentially relate to prostate tumor invasion and metastasis,” the researchers concluded. “Analysis of these genes provided insights into biological pathways relevant to the disease and disclosed innovative markers for diagnosis or prognosis as well as potential targets for therapy.”