The Prognostic Potential of Human Prostate Cancer-Associated Macrophage Subtypes as Revealed by Single-cell Transcriptomics

This article was originally published here

Mol Cancer Res. 2021 Jun 15:molcanres.0740.2020. doi: 10.1158/1541-7786.MCR-20-0740. Online ahead of print.

ABSTRACT

Macrophages in the tumor microenvironment are causally linked with prostate cancer development and progression, yet little is known about their composition in neoplastic human tissue. By performing single cell transcriptomic analysis of human prostate cancer resident macrophages, three distinct populations were identified in the diseased prostate. Unexpectedly, no differences were observed between macrophages isolated from the tumorous and non-tumorous portions of the prostatectomy specimens. Markers associated with canonical M1 and M2 macrophage phenotypes were identifiable, however these were not the main factors defining unique subtypes. The genes selectively associated with each macrophage cluster were used to develop a gene signature which was highly associated with both recurrence-free and metastasis-free survival. These results highlight the relevance of tissue-specific macrophage subtypes in the tumour microenvironment for prostate cancer progression and demonstrates the utility of profiling single-cell transcriptomics in human tumor samples as a strategy to design gene classifiers for patient prognostication. Implications: The specific macrophage subtypes present in a diseased human prostate have prognostic value, suggesting that the relative proportions of these populations are related to patient outcome. Understanding the relative contributions of these subtypes will not only inform patient prognostication, but will enable personalised immunotherapeutic strategies to increase beneficial populations or reduce detrimental populations.

PMID:34131070 | DOI:10.1158/1541-7786.MCR-20-0740