We report a robust, versatile approach named CRISPR Live-cell fluorescent in situ hybridization (LiveFISH) using fluorescent oligos for genome tracking in broad cell types including primary cells. An intrinsic stability switch of CRISPR guide RNAs enables LiveFISH to accurately detect chromosomal disorders such as Patau Syndrome in prenatal amniotic fluid cells and track multiple loci in human T lymphocytes. In addition, LiveFISH tracks the real-time movement of DNA double-strand breaks induced by CRISPR-Cas9-mediated editing and consequent chromosome translocations. Finally, combining Cas9 and Cas13 systems, LiveFISH allows for simultaneous visualization of genomic DNA and RNA transcripts in living cells. The LiveFISH approach enables real-time live imaging of DNA and RNA during genome editing, transcription, and rearrangements in single cells.
Today's Top 5
5. New Prosthetic Leg Combating Phantom Pain
4. Digital Health Flourishing Overseas
3. FDA Approves First Hypoglycemic Rescue Pen
2. Meru Health Offering "The New Standard of Mental Healthcare"
. @MeruHealth introduces 'The New Standard of Mental Healthcare' along with— Daniel Kraft, MD (@daniel_kraft) September 18, 2019
publication of peer-reviewed 1-year treatment In which enrolled patients had clinically significant reductions in symptoms of depression & anxiety https://t.co/7rgOpTg7p9 #digitalhealth #mentalhealth
1. Hydrogel Use in Modeling Cardiac Tissue
Hydrogel Mimics of Heart Tissue to Study Cardiac Reshaping Following Aortic Valve Implantation #Card #fitness #health #training #healthy #healthylifestyle #cardio #healthyliving #healthylife #medicine #healthcare #AI #cardioworkout #cardiologia #card https://t.co/jek10Y0bqm pic.twitter.com/UgLpzF9jV2— HeartIn 🗯️ (@HeartIn_net) September 20, 2019