We report a robust, versatile approach named CRISPR Live-cell fluorescent in situ hybridization (LiveFISH) using fluorescent oligos for genome tracking in broad cell types including primary cells. An intrinsic stability switch of CRISPR guide RNAs enables LiveFISH to accurately detect chromosomal disorders such as Patau Syndrome in prenatal amniotic fluid cells and track multiple loci in human T lymphocytes. In addition, LiveFISH tracks the real-time movement of DNA double-strand breaks induced by CRISPR-Cas9-mediated editing and consequent chromosome translocations. Finally, combining Cas9 and Cas13 systems, LiveFISH allows for simultaneous visualization of genomic DNA and RNA transcripts in living cells. The LiveFISH approach enables real-time live imaging of DNA and RNA during genome editing, transcription, and rearrangements in single cells.
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5. VR, AI and Robotics Improving Surgeries
#DigitalSurgery: The Robot Will Assist the Surgeon Now. @ShafiAhmed5 on the convergence of #AR, #VR #AI & #Robotics on augmenting the clinician of the future. https://t.co/hpWqv5D2Yw Join us next week for #xMed 2019. https://t.co/La9S00SM8Z #MedEd #hcldr #surgery #digitalHealth— Exponential Medicine (@ExponentialMed) October 30, 2019